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DIFF Channel

A surfactant in Stromatolyser-4DL causes lysis of red blood cells. At the same time, the cell membranes of white blood cells are slightly perforated.

The polymethine fluorescence marker Stromatolyser-4DS migrates into the WBC and binds in particular to nucleic acids and cell organelles. Additionally an organic acid in the Stromatolyser-4DL binds specifically to the granules in eosinophils as a result of which they can be differentiated from neutrophils by means of a higher side scatter signal.

Our XE- and XT-Series analysers provide five populations of white blood cells in the DIFF scattergram, including the Immature Granulocyte population.

The XS analysers’ scattergrams displays the following five populations: lymphocytes, monocytes, basophils, eosinophils and neutrophils. 

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