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NRBC channel

In the NRBC channel, a surfactant in the Stromatolyser-NR reagent lyses the red blood cell membranes, leaving only the nuclei of nucleated red blood cells (NRBC) intact. It also perforates the cell membrane of white blood cells, in which the polymethine fluorescence marker of stromatolyser-NR  labels the nucleic acids in the nuclei as well as cytoplasmic organelles.

NRBC, on the other hand, have lost their cytoplasm after the reaction so that the fluorescence marker only labels nucleic acids in the nucleus. This results in two distinct populations based on differences in fluorescence intensity. NRBC are separated from white blood cells due to their lower fluorescence intensity.

The white blood cell and lymphocyte count results are automatically corrected if NRBC are detected in the NRBC channel, prior to reporting. 

This channel is only available on XE-2100 and XE-5000.

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